Hexane fraction of Oldenlandiae diffusae Herba(ODH)which was being used for the treatmetn of caner in oriental medicine showed the best cytotoxicity against L1210 and A549 in the solvent fractions. Antitumor substance isolated from hexane
fraction
of
ODH was identified as ursolicacid(UA) by photometric analysis. IC50 of UA against cancer cells as SNU-1, HCT15, XF498, SK-MEL2 and Ab49 was 13㎍/㎖, 15㎍/㎖, 12㎍/㎖, 9㎍/㎖ and 11㎍/㎖respectively. It significantly inhibited the metastasis
tolungs
and
kidneys from pulmonary colonization assay and study on histological changes of organs and showed the enhancing effect on B cell dosage-dependently by FACS analysis.
T/C % of UA against S-180 cells was 171% % and its cytotoxicity against SNU-1 iant was confirmed from the morphological changes by elctronic microscopes such as SEM and TEM that it induced undulated memebrane 4 hr after UA treatment, and the
breakdown
of cell memebrane and nucleus 24 hr after UA treatment.
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